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KMID : 0616120160470020179
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Korean Journal of Pharmacognosy
2016 Volume.47 No. 2 p.179 ~ p.185
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Anti-oxidative Activity of Ethyl acetate Fraction of the Dried Ginger in Caenorhabditis elegans
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Lee Eun-Byeol
Kim Jun-Hyeong
Leem Jae-Yoon
Kim Hye-Soo
Kim Dae-Sung
Eun Jae-Soon
Han Soon-Cheon
Kim Dae-Keun
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Abstract
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inger (Zingiber officnale Roscoe, Zingiberaceae), which is a well-known food seasoning, has been used as a traditional medicine for the treatment of gastrointestinal disorder, vomiting and cough in Korea, China and Japan. Ethanol extract from the dried ginger (DG) was successively partitioned as methylene chloride, ethyl acetate, n-butanol and H2O soluble fractions. Among those fractions the ethyl acetate soluble fraction (EDG) showed the most potent DPPH radical scavenging and superoxide quenching activities. To know the effect of antioxidant activities of EDG, we tested the activities of superoxide dismutase (SOD) and catalase together with oxidative stress tolerance and intracellular ROS level in Caenorhabditis elegans. To investigate whether EDG-mediated increased stress tolerance was due to regulation of stress-response gene, we quantified SOD-3 expression using transgenic strain including CF1553. Consequently, EDG elevated SOD and catalase activities of C. elegans, reduced intracellular ROS accumulation in a dose?dependent manner. Moreover, EDG-treated CF1553 worms exhibited significantly higher SOD-3::GFP intensity.
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KEYWORD
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Zingiber officinale, Caenorhabditis elegans, SOD, Catalase, ROS
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